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KMID : 0545120020120050760
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 5 p.760 ~ p.765
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Production and Characterization of Chitosan from Ginseng-Steaming Effluents by Mucor miehei
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KIM, JAE HO
LEE, KI SUNG/KIM, NA MI/LEE, JONG SOO
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Abstract
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Mucor miehei KCTC6011, which grew successfully in ginseng-steaming effluents and produced a large amount of chitosan efficiently, was selected from various fungi. Approximately 120mg of chitosan per g-dry mycelium was maximally produced in 84 h at 25℃ when grown in the ginseng-steaming effluent (pH8.0) supplemented with 0.5% yeast extract and 0.002% CuSO_4. Chitosan produced by Mucor miehei KCTC6011 was identified by the IR-spectra to have deacetylated approximately 56%. Viscosity and molecular weight of the chitosan were 80 cps and 1.07×10^3 kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rizoctonia solani in 60h.
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KEYWORD
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